Core Projects

Z03: Proteomic and single-cell transcriptomic landscapes of cellular plasticity in myeloid malignancies

Summary

Transcriptomic, epigenomic and proteomic profiling are key technologies across the CRC to investigate and understand cell plasticity. Project Z03 will provide a technological platform for consistent and state-of-the-art proteome, single-cell transcriptome and chromatin accessibility analysis of cellular specimens collected from cell line systems, animal models, and patients under diverse types of treatment. Integration of multi-omics data from the CRC projects will provide an overarching view of common and shared regulatory programs that underlie cell plasticity allowing generation of a plasticity landscape for myeloid malignancies.

To achieve this, this project will closely collaborate with individual research projects in the CRC by the provision of SOPs for collection and processing of cellular specimens, by performing mass spectrometry-based analyses, by analysing proteomic and transcriptomic data and by integrating the data of multiple projects on CITE-Seq, proteomics and scATAC-Seq.

Lead Investigator