A03: Targeting metabolic plasticity to overcome chemotherapyresistance in acute myeloid leukemia

Project A03 will identify key metabolic features (e.g., OXPHOS, FAO, glycolysis, ROS, etc.) of primary AML cells that survived chemotherapy using metabolic spectral flow cytometry. Bone marrow organoids will be established to analyze metabolic adaptation of AML and niche cells under cytotoxic stress caused by clinically approved compounds. Single cell multi-omics, able to simultaneously determine metabolic properties, transcriptome and surface proteome will be applied in order to identify targetable vulnerabilities upon metabolic adaptation.